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Anions
Metrosep A Supp 3 - 250/4.6
The Metrosep A Supp 3 - 250/4.6 solves separation problems in aqueous and organic media. It can be used reliably with a wide range of eluents – even those with high proportions of organic solvents. With the Metrosep A Supp 3 - 250/4.6, samples which present a great analytical challenge can be analyzed in routine operation, for example the measurement of biological samples or the determination of inorganic anions in organic matrices. Polyphosphates can be separated effectively with the aid of a caustic soda gradient on the Metrosep A Supp 3 - 250/4.6. In isocratic operation, the column is also suitable for the separation of sulfite, sulfate and thiosulfate in less than 20 min.
The Metrosep A Supp 3 - 250/4.6 solves separation problems in aqueous and organic media. It can be used reliably with a wide range of eluents – even those with high proportions of organic solvents. With the Metrosep A Supp 3 - 250/4.6, samples which present a great analytical challenge can be analyzed in routine operation, for example the measurement of biological samples or the determination of inorganic anions in organic matrices. Polyphosphates can be separated effectively with the aid of a caustic soda gradient on the Metrosep A Supp 3 - 250/4.6. In isocratic operation, the column is also suitable for the separation of sulfite, sulfate and thiosulfate in less than 20 min.
| Column dimensions (mm) | 250 x 4.6 |
| Max. pressure | 20 MPa |
| Maximum flow | 2.5 mL/min |
| Standard flow | 1.0 mL/min |
| Housing material | PEEK |
| Carrier material | Polystyrene-divinylbenzene copolymer with quaternary ammonium groups |
| Particle size (µm) | 9 |
| Column type | 1 |
| pH range | 1...13 |
| Storage | In the eluent. For a longer period (weeks), store the column in a refrigerator at a minimum of +4 °C. |
| Weight | 0.23 |
| Regeneration | Rinse with 50 mL of a 0.05 mol/L solution of Na4EDTA at a flow of 0.5 mL/min. Afterwards, rinse with 0.1 mol/L NaOH at 0.5 mL/min for 1 h. |
| Application range | Applications
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| Capacity | 33 (µmol Cl–) |
| Organic modifier | 0 - 100% |
| Eluents | Carbonate eluent (standard eluent) Sodium bicarbonate: 1.7 mmol/L; 286 mg/2 L |
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